Home> Products > Animal Disease Testing Kits > Sheep Disease Detection Kit

Clostridium perfringens α Ab detection card

[Principle]
This reagent is prepared using the principle of colloidal gold immunoassay (GICA). After the sample is added to the sample well, it moves along the chromatography membrane with the colloidal gold marker. If there is Clostridium perfringens in the sample α Virus antibodies bind to colloidal gold markers and antigens on the detection line and display a purple red color. If there is no Clostridium perfringens in the sample α Virus antibodies do not produce color reactions.


Clostridium perfringens α Ab detection card (colloidal gold method)

[Packing Specifications] 40T / box

[Kit composition]


No.

Name

40T / box

1.

test   cards (including straws)

40

2.

Sample   dilution

40bottles

3.

straws

40 sticks

4.

instruction   manual

1

[Storage and expiry date] Store in a cool and dry place (2-30 ° C), valid for 24 months.



[Sample requirements]
1. This test card is only applicable for testing sheep serum/whole blood.
2. Serum: Extract 2-3ml of blood according to conventional methods and place it in a clean and dry test tube. Let it stand for about 1 hour until the blood clots, then centrifuge at 4000 rpm for 10 minutes (or let the blood stand for about 2 hours until the blood clots and naturally precipitates serum), and separate the serum. Require clear serum, no hemolysis, and no contamination. Serum samples can be stored at 2-8 ℃ in the short term, but need to be stored at -20 ℃ for the long term.

3. Whole blood: On the day fresh anticoagulant whole blood is collected, use it. Whole blood without anticoagulant is collected and used immediately.


[Test Method]
1. Serum/whole blood sampling method: Tear open the aluminum foil packaging bag of the test card, take out the test card, place it on a flat and clean table, use a straw inside the bag to slowly add 1 drop of blood serum or 2 drops of whole blood to the sampling hole, and then add 2 drops of diluent to the sampling hole.
2. Store at room temperature for 10-15 minutes to determine the result, and the color development result after 15 minutes is invalid.


【 Result judgment 】
1. Negative: Within the observation hole, only a purple red line appears in the control line area (C).
2. Positive: In the observation hole, both the detection line area (T) and the control line area (C) show a purple red line. The higher the antibody titer, the darker the color of the detection line (T).
3. Invalid: Within the observation hole, there is no purple red line in the control line area (C).